Scientific Director:  Kathleen Millen, PhD

Technical Director:  Linda Degenstein

Phone: 773-702-0688

Fax: 773-702-0271

Website:  http://osrf.bsd.uchicago.edu/facilities/transgenic_mouse.html

The UCCRC Transgenic Mouse/Embryonic Stem Cell Facility provides comprehensive genetic engineering services to alter the genome of the laboratory mouse.  Nine services provided by the Transgenic Mouse/Embryonic Stem Cell Facility are (1) transgenic mouse production from founder through F1 Stage; (2) ES cell technology mouse production; (3) ES cell gene targeting and culturing; (4) mouse embryonic feeder (MEF) cell production; (5) Timed pregnancies of various strains and lines of mice; (6) embryo rederivation; (7) ES cell line development; (8) various breeding services and GEM model line maintenance; and (9) DNA preparation from ES cell lines for the construction of a gene targeting construct.  In addition to the technical services, the Facility also offers assistance with the design of studies that require mouse molecular genetics and an annual course in Mouse Handling and Breeding.  This course covers information regarding analysis and handling of the mice once the Facility has generated them, as well as general breeding and handling procedures for mice. In providing these comprehensive services, the UCCRC Transgenic Mouse/Embryonic Stem Cell Facility has generated mouse models that have led to advances in our understanding of cancer, including cancers of the breast, skin, colon, brain and hematopoietic system.